Renewable algal photo H2 production without S control using acetate enriched fermenter effluents

Renewable energy production using microorganisms is one of the challenging issues for environmental sustainability. Algal hydrogen (H 2 ) has often been achieved by sulfur (S) and chloride ion (Cl ? deprivation in a growth medium; however, it may not be realistic to control S or Cl concentrations natural sources (e.g., wastewater). In this study, two different green algal species, Chlamydomonas reinhardtii Chlorella sorokiniana were selected their photosynthetic activities compared with acetate/Cl ratios both batch continuous modes. At 150 ratio, H rates 0.25–0.33 ?mol L ?1 min C. 0.20–0.38 , respectively. The hydrogenase (HydA) reactivation photosystem II (PSII) inhibitor test revealed that biohydrogen algae due activity. It was found maintaining greater than 60–100 leads O depletion thus renewable species. Molecular dynamics (MD) simulations bonding between Y z His 190 PSII supported inhibition acetate. Using fermenter effluents, showed successful ~80 ~95 respectively, 15 days. scavenging correlated -His interactions during cultivation investigated acetate under conditions, shown result subsequent photolysis. • impact ratio on studied. Acetate-rich wastewater potential scavenger without control. Continuous mode stabilizes sustaining ratio. will ensure